An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy

BACKGROUND: Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations. A rapid and reliable method of cloning native DNA sequences into desired plasmids are highly desired. RESULTS...

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Detalhes bibliográficos
Main Authors: Bi, Yanzhen, Qiao, Xianfeng, Hua, Zaidong, Zhang, Liping, Liu, Ximei, Li, Li, Hua, Wenjun, Xiao, Hongwei, Zhou, Jingrong, Wei, Qingxin, Zheng, Xinmin
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3408372/
https://ncbi.nlm.nih.gov/pubmed/22768962
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-39
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