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An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy
BACKGROUND: Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations. A rapid and reliable method of cloning native DNA sequences into desired plasmids are highly desired. RESULTS...
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| Main Authors: | , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3408372/ https://ncbi.nlm.nih.gov/pubmed/22768962 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-39 |
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