Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.

A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleo...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Kremsky, J N, Wooters, J L, Dougherty, J P, Meyers, R E, Collins, M, Brown, E L
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1987
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340705/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3562241/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.7.2891
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