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Chemical synthesis of oligonucleotides containing a free sulphydryl group and subsequent attachment of thiol specific probes.
Oligonucleotides containing a free sulphydryl group at their 5'-termini have been synthesised and further derivatised with thiol specific probes. The nucleotide sequence required is prepared using standard solid phase phosphoramidite techniques and an extra round of synthesis is then performed...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1985
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC321801/ https://ncbi.nlm.nih.gov/pubmed/4011448 |
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