Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.

A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splin...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Kremsky, J N, Wooters, J L, Dougherty, J P, Meyers, R E, Collins, M, Brown, E L
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1987
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340705/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3562241/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.7.2891
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