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Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.
A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleo...
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| Publicado en: | Nucleic Acids Res |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1987
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340705/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3562241/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.7.2891 |
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