In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase.
We describe a completely in vitro system for generating defined poliovirus proteinase mutations and subsequently assaying the phenotypic expression of such mutations. A complete cDNA copy of the entire poliovirus genome has been inserted into a bacteriophage T7 transcription vector. We have introduc...
Salvato in:
| Pubblicato in: | Nucleic Acids Res |
|---|---|
| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
1987
|
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340618/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3031587/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.5.2069 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|