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In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase.

We describe a completely in vitro system for generating defined poliovirus proteinase mutations and subsequently assaying the phenotypic expression of such mutations. A complete cDNA copy of the entire poliovirus genome has been inserted into a bacteriophage T7 transcription vector. We have introduc...

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Publicat a:Nucleic Acids Res
Autors principals: Ypma-Wong, M F, Semler, B L
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1987
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340618/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3031587/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.5.2069
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