In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase.
We describe a completely in vitro system for generating defined poliovirus proteinase mutations and subsequently assaying the phenotypic expression of such mutations. A complete cDNA copy of the entire poliovirus genome has been inserted into a bacteriophage T7 transcription vector. We have introduc...
Sparad:
| I publikationen: | Nucleic Acids Res |
|---|---|
| Huvudupphov: | , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Oxford University Press
1987
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| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340618/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3031587/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.5.2069 |
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