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In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase.
We describe a completely in vitro system for generating defined poliovirus proteinase mutations and subsequently assaying the phenotypic expression of such mutations. A complete cDNA copy of the entire poliovirus genome has been inserted into a bacteriophage T7 transcription vector. We have introduc...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1987
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC340618/ https://ncbi.nlm.nih.gov/pubmed/3031587 |
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