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Engineering an Improved IgG4 Molecule with Reduced Disulfide Bond Heterogeneity and Increased Fab Domain Thermal Stability

The integrity of antibody structure, stability, and biophysical characterization are becoming increasingly important as antibodies receive increasing scrutiny from regulatory authorities. We altered the disulfide bond arrangement of an IgG4 molecule by mutation of the Cys at the N terminus of the he...

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Detaylı Bibliyografya
Asıl Yazarlar: Peters, Shirley J., Smales, C. Mark, Henry, Alistair J., Stephens, Paul E., West, Shauna, Humphreys, David P.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Biochemistry and Molecular Biology 2012
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3397877/
https://ncbi.nlm.nih.gov/pubmed/22610095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.369744
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