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Watching DNA polymerase η make a phosphodiester bond
Native human DNA polymerase η, DNA and dATP were crystallized at pH 6.0 without Mg(2+). The polymerization reaction was initiated by exposing crystals to 1 mM Mg(2+) at pH 7.0, and stopped at various time points by freezing at 77 K for crystallographic analysis. The substrates and two Mg(2+) are ali...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3397672/ https://ncbi.nlm.nih.gov/pubmed/22785315 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature11181 |
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