Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy

We demonstrate how the resolution and imaging depth limitations of nonlinear optical microscopy can be overcome by modulating the spatial overlap between two-color pulses. We suppress out-of-focus signals, which limit the imaging depth, by a factor of 100, and enhance the lateral and axial resolutio...

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Bibliographic Details
Main Authors: Isobe, Keisuke, Kawano, Hiroyuki, Takeda, Takanori, Suda, Akira, Kumagai, Akiko, Mizuno, Hideaki, Miyawaki, Atsushi, Midorikawa, Katsumi
Format: Artigo
Language:Inglês
Published: Optical Society of America 2012
Subjects:
Microscopy
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3395484/
https://ncbi.nlm.nih.gov/pubmed/22808431
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.3.001594
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