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Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy

We demonstrate how the resolution and imaging depth limitations of nonlinear optical microscopy can be overcome by modulating the spatial overlap between two-color pulses. We suppress out-of-focus signals, which limit the imaging depth, by a factor of 100, and enhance the lateral and axial resolutio...

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Detalhes bibliográficos
Main Authors: Isobe, Keisuke, Kawano, Hiroyuki, Takeda, Takanori, Suda, Akira, Kumagai, Akiko, Mizuno, Hideaki, Miyawaki, Atsushi, Midorikawa, Katsumi
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3395484/
https://ncbi.nlm.nih.gov/pubmed/22808431
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.3.001594
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