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Combining in situ proteolysis and mass spectrometry to crystallize Escherichia coli PgaB
The periplasmic poly-β-1,6-N-acetyl-d-glucosamine (PNAG) de-N-acetylase PgaB from Escherichia coli was overexpressed and purified, but was recalcitrant to crystallization. Use of the in situ proteolysis technique produced crystals of PgaB, but these crystals could not be optimized for diffraction st...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3388937/ https://ncbi.nlm.nih.gov/pubmed/22750880 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112022075 |
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