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Mass Spectrometry Guided In Situ Proteolysis to Obtain Crystals for X-ray Structure Determination

A strategy for increasing the efficiency of protein crystallization/structure determination with mass spectrometry has been developed. This approach combines insights from limited proteolysis/mass spectrometry and crystallization via in situ proteolysis. The procedure seeks to identify protease-resi...

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Detalhes bibliográficos
Main Authors: Gheyi, Tarun, Rodgers, Logan, Romero, Richard, Sauder, J. Michael, Burley, Stephen K.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2963156/
https://ncbi.nlm.nih.gov/pubmed/20685133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2010.06.015
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