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In situ proteolysis for protein crystallization and structure determination
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at c...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3366506/ https://ncbi.nlm.nih.gov/pubmed/17982461 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth1118 |
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