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‘Seeding’ with protease to optimize protein crystallization conditions in in situ proteolysis
In situ proteolysis is one of the most effective rescue strategies for protein crystallization, and optimization of the ratio between the protein and the protease is one of the key steps in the process. Seeding is a very powerful tool to optimize crystallization conditions and can be performed by mo...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3374523/ https://ncbi.nlm.nih.gov/pubmed/22691798 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911201161X |
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