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‘Seeding’ with protease to optimize protein crystallization conditions in in situ proteolysis

In situ proteolysis is one of the most effective rescue strategies for protein crystallization, and optimization of the ratio between the protein and the protease is one of the key steps in the process. Seeding is a very powerful tool to optimize crystallization conditions and can be performed by mo...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Huang, Jinguang, Gong, Yanmei, Huang, Dan, Haire, Lesley, Liu, Junfeng, Peng, Youliang
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: International Union of Crystallography 2012
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3374523/
https://ncbi.nlm.nih.gov/pubmed/22691798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430911201161X
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