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In Situ Proteolysis to Generate Crystals for Structure Determination: An Update

For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, and among these only 13–15 crystallize in a form that enables structure determination. In 2007, Dong et al reported that the addition of trace amounts of protease to crystallization trials—in situ prot...

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Detalhes bibliográficos
Main Authors: Wernimont, Amy, Edwards, Aled
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2661377/
https://ncbi.nlm.nih.gov/pubmed/19352432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0005094
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