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In situ proteolysis, crystallization and preliminary X-ray diffraction analysis of a V(H)H that binds listeria internalin B

The variable region of camelid heavy-chain antibodies produces the smallest known antibody fragment with antigen-binding capability (a V(H)H). The V(H)H R303 binds internalin B (InlB), a virulence factor expressed by the pathogen Listeria monocytogenes. InlB is critical for initiation of Listeria in...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Huh, Ian, Gene, Robert, Kumaran, Jyothi, MacKenzie, C. Roger, Brooks, Cory L.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4231859/
https://ncbi.nlm.nih.gov/pubmed/25372824
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X1402010X
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