Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates.

The direct sequencing of DNA generated by the polynucleotide chain reaction, via the incorporation of phosphorothioate nucleotides and followed by treatment with an alkylating reagent that cleaves specifically at the phosphorothioate positions, is described. The Taq polymerase used in the amplificat...

Celý popis

Podrobná bibliografie

Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Nakamaye, K L, Gish, G, Eckstein, F, Vosberg, H P
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 1988
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338829/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3194230/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.21.9947
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!