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Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates.

The direct sequencing of DNA generated by the polynucleotide chain reaction, via the incorporation of phosphorothioate nucleotides and followed by treatment with an alkylating reagent that cleaves specifically at the phosphorothioate positions, is described. The Taq polymerase used in the amplificat...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
मुख्य लेखकों: Nakamaye, K L, Gish, G, Eckstein, F, Vosberg, H P
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: 1988
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC338829/
https://ncbi.nlm.nih.gov/pubmed/3194230
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