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T7 RNA Polymerase Functions In Vitro without Clustering

Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using ‘pulldowns’ and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)<1 µM. C...

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Bibliografiske detaljer
Main Authors: Finan, Kieran, Torella, Joseph P., Kapanidis, Achillefs N., Cook, Peter R.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388079/
https://ncbi.nlm.nih.gov/pubmed/22768341
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0040207
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