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T7 RNA Polymerase Functions In Vitro without Clustering
Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using ‘pulldowns’ and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)<1 µM. C...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3388079/ https://ncbi.nlm.nih.gov/pubmed/22768341 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0040207 |
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