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Efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons

The derivation of germ-line competent avian primordial germ cells establishes a cell-based model system for the investigation of germ cell differentiation and the production of genetically modified animals. Current methods to modify primordial germ cells using DNA or retroviral vectors are inefficie...

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Bibliografische gegevens
Hoofdauteurs: Macdonald, Joni, Taylor, Lorna, Sherman, Adrian, Kawakami, Koichi, Takahashi, Yoshiko, Sang, Helen M., McGrew, Michael J.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 2012
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3384192/
https://ncbi.nlm.nih.gov/pubmed/22586100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1118715109
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