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Efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons

The derivation of germ-line competent avian primordial germ cells establishes a cell-based model system for the investigation of germ cell differentiation and the production of genetically modified animals. Current methods to modify primordial germ cells using DNA or retroviral vectors are inefficie...

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Autors principals: Macdonald, Joni, Taylor, Lorna, Sherman, Adrian, Kawakami, Koichi, Takahashi, Yoshiko, Sang, Helen M., McGrew, Michael J.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3384192/
https://ncbi.nlm.nih.gov/pubmed/22586100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1118715109
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