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Efficient genetic modification and germ-line transmission of primordial germ cells using piggyBac and Tol2 transposons
The derivation of germ-line competent avian primordial germ cells establishes a cell-based model system for the investigation of germ cell differentiation and the production of genetically modified animals. Current methods to modify primordial germ cells using DNA or retroviral vectors are inefficie...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3384192/ https://ncbi.nlm.nih.gov/pubmed/22586100 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1118715109 |
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