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Affinity resins containing enzymatically resistant mRNA cap analogs—a new tool for the analysis of cap-binding proteins
Cap-binding proteins have been routinely isolated using m(7)GTP-Sepharose; however, this resin is inefficient for proteins such as DcpS (scavenger decapping enzyme), which interacts not only with the 7-methylguanosine, but also with the second cap base. In addition, DcpS purification may be hindered...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Cold Spring Harbor Laboratory Press
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3383972/ https://ncbi.nlm.nih.gov/pubmed/22589334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.032078.111 |
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