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Induced heterodimerization and purification of two target proteins by a synthetic coiled-coil tag
A synthetic de novo designed heterodimeric coiled-coil was used to copurify two target fluorescent proteins, Venus and enhanced cyan fluorescent protein (ECFP). The coiled-coil consists of two 21-amino acid repetitive sequences, (EIAALEK)(3) and (KIAALKE)(3), named E3 and K3, respectively. These seq...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Wiley Subscription Services, Inc., A Wiley Company
2012
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3375751/ https://ncbi.nlm.nih.gov/pubmed/22362668 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2035 |
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