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Inexpensive synthetic-based matrix for both conventional and rapid purification of protein A- and tandem affinity purification-tagged proteins

Immunoglobulin G (IgG)–Sepharose is often used for purification of protein A- and tandem affinity purification (TAP)-tagged proteins from eukaryotic cells, but because it is based on an agarose matrix, it is not always optimal for all proteins. Synthetic matrices such as IgG–Dynabeads have improved...

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Hlavní autoři: Sawin, Kenneth E., Bicho, Claudia C., Snaith, Hilary A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Academic Press 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2810510/
https://ncbi.nlm.nih.gov/pubmed/19799851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.09.045
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