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Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag
The development of affinity tags has greatly simplified protein purification procedures. A variety of affinity tags are now available to improve expression, solubility, and/or tag removal. In this chapter, we describe a method for purifying recombinant proteins expressed in Escherichia coli that use...
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| Udgivet i: | Methods Mol Biol |
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| Hovedforfatter: | |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4377278/ https://ncbi.nlm.nih.gov/pubmed/24943314 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1034-2_5 |
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