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Dda helicase tightly couples translocation on single-stranded DNA to unwinding of duplex DNA: Dda is an optimally active helicase

Helicases utilize the energy of ATP hydrolysis to unwind double-stranded DNA while translocating on the DNA. Mechanisms for melting the duplex have been characterized as active or passive, depending on whether the enzyme actively separates the base pairs or simply sequesters single-stranded DNA that...

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Bibliographic Details
Main Authors: Byrd, Alicia K., Matlock, Dennis L., Bagchi, Debjani, Aarattuthodiyil, Suja, Harrison, David, Croquette, Vincent, Raney, Kevin D.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3372602/
https://ncbi.nlm.nih.gov/pubmed/22504228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.04.007
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