Yeast Pif1 Helicase Exhibits a One-base-pair Stepping Mechanism for Unwinding Duplex DNA

Kinetic analysis of the DNA unwinding and translocation activities of helicases is necessary for characterization of the biochemical mechanism(s) for this class of enzymes. Saccharomyces cerevisiae Pif1 helicase was characterized using presteady state kinetics to determine rates of DNA unwinding, di...

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Main Authors: Ramanagoudr-Bhojappa, Ramanagouda, Chib, Shubeena, Byrd, Alicia K., Aarattuthodiyil, Suja, Pandey, Manjula, Patel, Smita S., Raney, Kevin D.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Biochemistry and Molecular Biology 2013
Fag:
DNA and Chromosomes
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3668774/
https://ncbi.nlm.nih.gov/pubmed/23596008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.470013
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