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Yeast Pif1 Helicase Exhibits a One-base-pair Stepping Mechanism for Unwinding Duplex DNA

Kinetic analysis of the DNA unwinding and translocation activities of helicases is necessary for characterization of the biochemical mechanism(s) for this class of enzymes. Saccharomyces cerevisiae Pif1 helicase was characterized using presteady state kinetics to determine rates of DNA unwinding, di...

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Main Authors: Ramanagoudr-Bhojappa, Ramanagouda, Chib, Shubeena, Byrd, Alicia K., Aarattuthodiyil, Suja, Pandey, Manjula, Patel, Smita S., Raney, Kevin D.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Biochemistry and Molecular Biology 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3668774/
https://ncbi.nlm.nih.gov/pubmed/23596008
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.470013
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