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Ring-opened 7-methylguanine residues in DNA are a block to in vitro DNA synthesis.
Single-stranded M13mp18 phage DNA was methylated with dimethylsulfate (DMS), and further treated with alkali to ring-open N7-methylguanine residues and yield 2-6-diamino-4-hydroxy-5N-methylformamidopyrimidine (Fapy) residues. Nucleotide incorporation during in vitro DNA synthesis on methylated templ...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1988
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC336835/ https://ncbi.nlm.nih.gov/pubmed/3399381 |
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