Identification of non-specific hybridization using an empirical equation fitted to non-equilibrium dissociation curves

Non-equilibrium dissociation curves (NEDCs) have the potential to identify non-specific hybridizations on high throughput, diagnostic microarrays. We report a simple method for identification of non-specific signals by using a new parameter that does not rely on comparison of perfect match and misma...

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Bibliographic Details
Main Authors: Baushke, Samuel W, Stedtfeld, Robert D, Tourlousse, Dieter M, Ahmad, Farhan, Wick, Lukas M, Gulari, Erdogan, Tiedje, James M, Hashsham, Syed A
Format: Artigo
Language:Inglês
Published: 2012
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3366151/
https://ncbi.nlm.nih.gov/pubmed/22537822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2012.04.003
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