Development and Experimental Validation of a Predictive Threshold Cycle Equation for Quantification of Virulence and Marker Genes by High-Throughput Nanoliter-Volume PCR on the OpenArray Platform

Development of quantitative PCR (QPCR) assays typically requires extensive screening within and across a given species to ensure specific detection and lucid identification among various pathogenic and nonpathogenic strains and to generate standard curves. To minimize screening requirements, multipl...

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Bibliographic Details
Main Authors: Stedtfeld, Robert D., Baushke, Samuel W., Tourlousse, Dieter M., Miller, Sarah M., Stedtfeld, Tiffany M., Gulari, Erdogan, Tiedje, James M., Hashsham, Syed A.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 2008
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2446556/
https://ncbi.nlm.nih.gov/pubmed/18424532
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02743-07
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