Influence of Dangling Ends and Surface-Proximal Tails of Targets on Probe-Target Duplex Formation in 16S rRNA Gene-Based Diagnostic Arrays

Dangling ends and surface-proximal tails of gene targets influence probe-target duplex formation and affect the signal intensity of probes on diagnostic microarrays. This phenomenon was evaluated using an oligonucleotide microarray containing 18-mer probes corresponding to the 16S rRNA genes of 10 w...

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Detalhes bibliográficos
Main Authors: Stedtfeld, Robert D., Wick, Lukas M., Baushke, Samuel W., Tourlousse, Dieter M., Herzog, Amanda B., Xia, Yongmei, Rouillard, Jean Marie, Klappenbach, Joel A., Cole, James R., Gulari, Erdogan, Tiedje, James M., Hashsham, Syed A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1796975/
https://ncbi.nlm.nih.gov/pubmed/17114322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01785-06
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