The cloning, purification and characterization of the Eco RV modification methylase.

The gene for the Eco RV methylase has been cloned into a plasmid under control of the strong lambda PL promoter and overexpressed in E. coli. This plasmid, pVIC1, gives reliable overexpression of the methylase at levels of about 20% of total protein. Maximum yields of soluble protein are achieved af...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	Nucleic Acids Res
Päätekijät:	Nwosu, V U, Connolly, B A, Halford, S E, Garnett, J
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	Oxford University Press 1988
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336551/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2836798/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.9.3705
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