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Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Nelson, M, Christ, C, Schildkraut, I
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1984
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318911/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6087274/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.13.5165
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