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Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I...

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Main Authors: Nelson, M, Christ, C, Schildkraut, I
Formato: Artigo
Idioma:Inglês
Publicado: 1984
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC318911/
https://ncbi.nlm.nih.gov/pubmed/6087274
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