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Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Principais autores: Nelson, M, Christ, C, Schildkraut, I
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1984
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318911/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6087274/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/12.13.5165
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