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The cloning, purification and characterization of the Eco RV modification methylase.
The gene for the Eco RV methylase has been cloned into a plasmid under control of the strong lambda PL promoter and overexpressed in E. coli. This plasmid, pVIC1, gives reliable overexpression of the methylase at levels of about 20% of total protein. Maximum yields of soluble protein are achieved af...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1988
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC336551/ https://ncbi.nlm.nih.gov/pubmed/2836798 |
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