FRET-Based Identification of mRNAs Undergoing Translation

We present proof-of-concept in vitro results demonstrating the feasibility of using single molecule fluorescence resonance energy transfer (smFRET) measurements to distinguish, in real time, between individual ribosomes programmed with several different, short mRNAs. For these measurements we use ei...

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Main Authors: Stevens, Benjamin, Chen, Chunlai, Farrell, Ian, Zhang, Haibo, Kaur, Jaskiran, Broitman, Steven L., Smilansky, Zeev, Cooperman, Barry S., Goldman, Yale E.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2012
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3365013/
https://ncbi.nlm.nih.gov/pubmed/22693619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0038344
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