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FRET-Based Identification of mRNAs Undergoing Translation

We present proof-of-concept in vitro results demonstrating the feasibility of using single molecule fluorescence resonance energy transfer (smFRET) measurements to distinguish, in real time, between individual ribosomes programmed with several different, short mRNAs. For these measurements we use ei...

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Bibliografiske detaljer
Main Authors: Stevens, Benjamin, Chen, Chunlai, Farrell, Ian, Zhang, Haibo, Kaur, Jaskiran, Broitman, Steven L., Smilansky, Zeev, Cooperman, Barry S., Goldman, Yale E.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3365013/
https://ncbi.nlm.nih.gov/pubmed/22693619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0038344
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