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Solid phase DNA sequencing using the biotin-avidin system.

A novel method for solid-phase DNA sequencing is described. A plasmid vector, pRIT27, has been designed to allow directional immobilization of double stranded plasmid to avidin agarose. The strategy involves enzymatic incorporation of 11-bio-dUTP into the plasmid and strand specific elution using al...

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Bibliografske podrobnosti
Main Authors: Ståhl, S, Hultman, T, Olsson, A, Moks, T, Uhlén, M
Format: Artigo
Jezik:Inglês
Izdano: 1988
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC336449/
https://ncbi.nlm.nih.gov/pubmed/3368314
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