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DNA orientation using specific avidin-ferritin biotin end labelling.

The orientation of DNA molecules has been determined by labelling one of the molecule end with a Biotin-labelled analog of dTTP (Bio-dUTP) and then by complexing the Bio-dUTP with Avidin-Ferritin. DNAs of phi X174, pBR322 and SV40 were end labelled with Bio-dUTP and imaged by Electron Microscopy (EM...

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Bibliografske podrobnosti
Main Authors: Theveny, B, Revet, B
Format: Artigo
Jezik:Inglês
Izdano: 1987
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC340500/
https://ncbi.nlm.nih.gov/pubmed/3029711
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