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Solid phase DNA sequencing using the biotin-avidin system.
A novel method for solid-phase DNA sequencing is described. A plasmid vector, pRIT27, has been designed to allow directional immobilization of double stranded plasmid to avidin agarose. The strategy involves enzymatic incorporation of 11-bio-dUTP into the plasmid and strand specific elution using al...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1988
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| オンライン・アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336449/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3368314/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.7.3025 |
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