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Solid phase DNA sequencing using the biotin-avidin system.
A novel method for solid-phase DNA sequencing is described. A plasmid vector, pRIT27, has been designed to allow directional immobilization of double stranded plasmid to avidin agarose. The strategy involves enzymatic incorporation of 11-bio-dUTP into the plasmid and strand specific elution using al...
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| Main Authors: | , , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1988
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC336449/ https://ncbi.nlm.nih.gov/pubmed/3368314 |
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