Solid phase DNA sequencing using the biotin-avidin system.

A novel method for solid-phase DNA sequencing is described. A plasmid vector, pRIT27, has been designed to allow directional immobilization of double stranded plasmid to avidin agarose. The strategy involves enzymatic incorporation of 11-bio-dUTP into the plasmid and strand specific elution using al...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Ståhl, S, Hultman, T, Olsson, A, Moks, T, Uhlén, M
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1988
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336449/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3368314/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.7.3025
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