Accurate in vitro cleavage by RNase III of phosphorothioate-substituted RNA processing signals in bacteriophage T7 early mRNA.
To test the ability of an RNA processing enzyme to cleave chemically-modified RNA substrates, RNA transcripts containing RNase III cleavage sites were enzymatically synthesized in vitro to contain specific phosphorothioate diester internucleotide linkages. One transcript (R1.1 RNA) was generated usi...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1988
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| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336336/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3279395/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.4.1577 |
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