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Accurate in vitro cleavage by RNase III of phosphorothioate-substituted RNA processing signals in bacteriophage T7 early mRNA.

To test the ability of an RNA processing enzyme to cleave chemically-modified RNA substrates, RNA transcripts containing RNase III cleavage sites were enzymatically synthesized in vitro to contain specific phosphorothioate diester internucleotide linkages. One transcript (R1.1 RNA) was generated usi...

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Udgivet i:Nucleic Acids Res
Principais autores: Nicholson, A W, Niebling, K R, McOsker, P L, Robertson, H D
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1988
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336336/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3279395/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.4.1577
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