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Efficient correction of a mutation by use of chemically synthesized DNA.
The mutated base in the am3 lysis-defective mutant of the bacteriophage phiX174 has been corrected by a combined in vitro enzymatic DNA synthesis and in vivo replication of the heteroduplex product. Chemically synthesized oligodeoxyribonucleotides carrying the wild-type sequence have been used to pr...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1978
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336094/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/279913/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.9.4268 |
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