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In vivo structure–function analysis of human Dicer reveals directional processing of precursor miRNAs

Dicer is an RNase III family endoribonuclease and haploinsufficient tumor suppressor that processes mature miRNAs from the 5′ (5p) or 3′ (3p) arm of hairpin precursors. In murine Dicer knockout fibroblasts, we expressed human Dicer with point mutations in the RNase III, helicase, and PAZ domains and...

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Bibliografiska uppgifter
Huvudupphovsmän: Gurtan, Allan M., Lu, Victoria, Bhutkar, Arjun, Sharp, Phillip A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Cold Spring Harbor Laboratory Press 2012
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3358635/
https://ncbi.nlm.nih.gov/pubmed/22546613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.032680.112
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